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Elabscience Biotechnology human tff3 elisa kit

<t>TFF3</t> has a high secretion level in the colorectal tumor conditional medium. A Gene expression level related to the function of CD8 + T cells (mean ± SD, n = 4). B The differentially expressed proteins between the CM and TCM groups. C Subcellular localization of differentially expressed proteins. D The most differentially expressed extracellular secreted proteins. E. The secretion level of TFF3 (mean ± SD, n = 6)

Human Tff3 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+tff3+elisa+kit/pmc12325462-33-18-22?v=Elabscience+Biotechnology
Average 93 stars, based on 4 article reviews

human tff3 elisa kit - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer"

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

Journal: International Journal of Colorectal Disease

doi: 10.1007/s00384-025-04964-0

TFF3 has a high secretion level in the colorectal tumor conditional medium. A Gene expression level related to the function of CD8 + T cells (mean ± SD, n = 4). B The differentially expressed proteins between the CM and TCM groups. C Subcellular localization of differentially expressed proteins. D The most differentially expressed extracellular secreted proteins. E. The secretion level of TFF3 (mean ± SD, n = 6)

Figure Legend Snippet: TFF3 has a high secretion level in the colorectal tumor conditional medium. A Gene expression level related to the function of CD8 + T cells (mean ± SD, n = 4). B The differentially expressed proteins between the CM and TCM groups. C Subcellular localization of differentially expressed proteins. D The most differentially expressed extracellular secreted proteins. E. The secretion level of TFF3 (mean ± SD, n = 6)

Techniques Used: Gene Expression

TFF3 affects the infiltration and function of CD8 + T cells into colorectal tumor. A The immune infiltration in tumor tissues of all patients from the TCGA-COAD database. B The correlation of immune cells from the TCGA-COAD database. C Analysis of immune cell infiltration in tumor samples with varying levels of TFF3 expression. D The level of GZMB and PRF1 based on the different expression of TFF3. E The secretory level of TFF3 after knockdown (mean ± SD, n = 4). F The expression level of GZMB, TNF, and IFNG after the knockdown of TFF3 (mean ± SD, n = 3)

Figure Legend Snippet: TFF3 affects the infiltration and function of CD8 + T cells into colorectal tumor. A The immune infiltration in tumor tissues of all patients from the TCGA-COAD database. B The correlation of immune cells from the TCGA-COAD database. C Analysis of immune cell infiltration in tumor samples with varying levels of TFF3 expression. D The level of GZMB and PRF1 based on the different expression of TFF3. E The secretory level of TFF3 after knockdown (mean ± SD, n = 4). F The expression level of GZMB, TNF, and IFNG after the knockdown of TFF3 (mean ± SD, n = 3)

Techniques Used: Expressing, Knockdown

Knockdown of TFF3 restricted tumor growth. A Tumor growth curve of CT-26 inoculated with control (shNC) or shTFF3 tumor cells (mean ± SEM, n = 6). B The tumor was excised from the mice at the time of euthanasia. C The concentration of TFF3 from tumor interstitial fluid ( n = 3). D The infiltration of CD8 + and CD4 + T cells in the mice tumor. E Flow cytometry analysis of the immune infiltration ( n = 5). F The secretory level of TNF and GZMB in mouse serum (mean ± SD, n = 4)

Figure Legend Snippet: Knockdown of TFF3 restricted tumor growth. A Tumor growth curve of CT-26 inoculated with control (shNC) or shTFF3 tumor cells (mean ± SEM, n = 6). B The tumor was excised from the mice at the time of euthanasia. C The concentration of TFF3 from tumor interstitial fluid ( n = 3). D The infiltration of CD8 + and CD4 + T cells in the mice tumor. E Flow cytometry analysis of the immune infiltration ( n = 5). F The secretory level of TNF and GZMB in mouse serum (mean ± SD, n = 4)

Techniques Used: Knockdown, Control, Concentration Assay, Flow Cytometry

Knockdown of TFF3 increases the infiltration of CD8 + T cells in vivo. A Schematic diagram for RNA-seq of tumor cells and T cells sorted from CT-26 tumors formed by shNC and shTFF3 tumor cells. B Detection of CD8 + T cells in tumors with immunofluorescence. Scale bars represent 50 µm (mean ± SD, n = 3). C Volcano plot of differentially expressed genes for TIL-T. D KEGG enrichment analysis of differentially expressed genes for TIL-T

Figure Legend Snippet: Knockdown of TFF3 increases the infiltration of CD8 + T cells in vivo. A Schematic diagram for RNA-seq of tumor cells and T cells sorted from CT-26 tumors formed by shNC and shTFF3 tumor cells. B Detection of CD8 + T cells in tumors with immunofluorescence. Scale bars represent 50 µm (mean ± SD, n = 3). C Volcano plot of differentially expressed genes for TIL-T. D KEGG enrichment analysis of differentially expressed genes for TIL-T

Techniques Used: Knockdown, In Vivo, RNA Sequencing, Immunofluorescence

Knockdown of TFF3 reduces the expression level of PD-L1 on tumor. A The KEGG enrichment analysis on immune infiltration-related genes was performed by the Cytoscape software. B The expression level of PD-L1 in tumor tissues (mean ± SD, n = 3). C Genes participated in the PD-L1 expression and PD-1 checkpoint pathway in cancer

Figure Legend Snippet: Knockdown of TFF3 reduces the expression level of PD-L1 on tumor. A The KEGG enrichment analysis on immune infiltration-related genes was performed by the Cytoscape software. B The expression level of PD-L1 in tumor tissues (mean ± SD, n = 3). C Genes participated in the PD-L1 expression and PD-1 checkpoint pathway in cancer

Techniques Used: Knockdown, Expressing, Software

Knocking down TFF3 inhibits the AKT/mTOR pathway. A The expression level of p-AKT/AKT (mean ± SD, n = 3). B The expression level of p-mTOR/mTOR (mean ± SD, n = 3). C Validation of the interaction between TFF3 and AKT by Co-IP. D The construction of CEA-CAR. E The apoptosis rates of DLD1 shTFF3 and DLD1 shNC cancer cell lines (mean ± SD, n = 7)

Figure Legend Snippet: Knocking down TFF3 inhibits the AKT/mTOR pathway. A The expression level of p-AKT/AKT (mean ± SD, n = 3). B The expression level of p-mTOR/mTOR (mean ± SD, n = 3). C Validation of the interaction between TFF3 and AKT by Co-IP. D The construction of CEA-CAR. E The apoptosis rates of DLD1 shTFF3 and DLD1 shNC cancer cell lines (mean ± SD, n = 7)

Techniques Used: Expressing, Biomarker Discovery, Co-Immunoprecipitation Assay

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Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: TFF3 has a high secretion level in the colorectal tumor conditional medium. A Gene expression level related to the function of CD8 + T cells (mean ± SD, n = 4). B The differentially expressed proteins between the CM and TCM groups. C Subcellular localization of differentially expressed proteins. D The most differentially expressed extracellular secreted proteins. E. The secretion level of TFF3 (mean ± SD, n = 6)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Gene Expression

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: TFF3 affects the infiltration and function of CD8 + T cells into colorectal tumor. A The immune infiltration in tumor tissues of all patients from the TCGA-COAD database. B The correlation of immune cells from the TCGA-COAD database. C Analysis of immune cell infiltration in tumor samples with varying levels of TFF3 expression. D The level of GZMB and PRF1 based on the different expression of TFF3. E The secretory level of TFF3 after knockdown (mean ± SD, n = 4). F The expression level of GZMB, TNF, and IFNG after the knockdown of TFF3 (mean ± SD, n = 3)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Expressing, Knockdown

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 restricted tumor growth. A Tumor growth curve of CT-26 inoculated with control (shNC) or shTFF3 tumor cells (mean ± SEM, n = 6). B The tumor was excised from the mice at the time of euthanasia. C The concentration of TFF3 from tumor interstitial fluid ( n = 3). D The infiltration of CD8 + and CD4 + T cells in the mice tumor. E Flow cytometry analysis of the immune infiltration ( n = 5). F The secretory level of TNF and GZMB in mouse serum (mean ± SD, n = 4)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, Control, Concentration Assay, Flow Cytometry

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 increases the infiltration of CD8 + T cells in vivo. A Schematic diagram for RNA-seq of tumor cells and T cells sorted from CT-26 tumors formed by shNC and shTFF3 tumor cells. B Detection of CD8 + T cells in tumors with immunofluorescence. Scale bars represent 50 µm (mean ± SD, n = 3). C Volcano plot of differentially expressed genes for TIL-T. D KEGG enrichment analysis of differentially expressed genes for TIL-T

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, In Vivo, RNA Sequencing, Immunofluorescence

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knockdown of TFF3 reduces the expression level of PD-L1 on tumor. A The KEGG enrichment analysis on immune infiltration-related genes was performed by the Cytoscape software. B The expression level of PD-L1 in tumor tissues (mean ± SD, n = 3). C Genes participated in the PD-L1 expression and PD-1 checkpoint pathway in cancer

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Knockdown, Expressing, Software

Journal: International Journal of Colorectal Disease

Article Title: Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer

doi: 10.1007/s00384-025-04964-0

Figure Lengend Snippet: Knocking down TFF3 inhibits the AKT/mTOR pathway. A The expression level of p-AKT/AKT (mean ± SD, n = 3). B The expression level of p-mTOR/mTOR (mean ± SD, n = 3). C Validation of the interaction between TFF3 and AKT by Co-IP. D The construction of CEA-CAR. E The apoptosis rates of DLD1 shTFF3 and DLD1 shNC cancer cell lines (mean ± SD, n = 7)

Article Snippet: We collected the supernatant from NCM460, HT29, and DLD1 cell lines to verify the proteomics results with a human TFF3 ELISA kit (Elabscience).

Techniques: Expressing, Biomarker Discovery, Co-Immunoprecipitation Assay

Classification performances and feature selection with XGBoost in distinguishing between PD, PSP and HC.

Journal: International Journal of Molecular Sciences

Article Title: Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson’s Disease and Progressive Supranuclear Palsy

doi: 10.3390/ijms252111663

Figure Lengend Snippet: Classification performances and feature selection with XGBoost in distinguishing between PD, PSP and HC.

Article Snippet: Human TFF3 Quantikine ELISA kit (DTFF30) was provided by R&D Systems (Minneapolis, MN, USA) while human CPB1 and OPG ELISA 96-assays (EH67RB and EHTNFRSF11B, respectively) were provided by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Selection

Serum concentration of TFF3 ( A ), CPB1 ( B ), and OPG ( C ) in PD (n = 46), PSP (n = 30) and HC (n = 24), as measured by ELISA. In each box plot, the 25th percentile, 75th percentile, and median of data are depicted as lower, upper, and middle lines, respectively, while bars on vertical lines indicate ranges. The Kruskal–Wallis test was used to calculate shown p-values. TFF3 = trefoil factor 3; CPB1 = carboxypeptidase B1; OPG = osteoprotegerin; PD = Parkinson’s disease; PSP = progressive supranuclear palsy; HC = healthy controls.

Journal: International Journal of Molecular Sciences

Article Title: Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson’s Disease and Progressive Supranuclear Palsy

doi: 10.3390/ijms252111663

Figure Lengend Snippet: Serum concentration of TFF3 ( A ), CPB1 ( B ), and OPG ( C ) in PD (n = 46), PSP (n = 30) and HC (n = 24), as measured by ELISA. In each box plot, the 25th percentile, 75th percentile, and median of data are depicted as lower, upper, and middle lines, respectively, while bars on vertical lines indicate ranges. The Kruskal–Wallis test was used to calculate shown p-values. TFF3 = trefoil factor 3; CPB1 = carboxypeptidase B1; OPG = osteoprotegerin; PD = Parkinson’s disease; PSP = progressive supranuclear palsy; HC = healthy controls.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Linear regression analysis between serum protein levels, demographic variables, and clinical variables in PD patients.

Journal: International Journal of Molecular Sciences

Article Title: Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson’s Disease and Progressive Supranuclear Palsy

doi: 10.3390/ijms252111663

Figure Lengend Snippet: Linear regression analysis between serum protein levels, demographic variables, and clinical variables in PD patients.

Techniques:

Linear regression analysis between serum protein levels, demographic and clinical variables in PSP patients.

Journal: International Journal of Molecular Sciences

Article Title: Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson’s Disease and Progressive Supranuclear Palsy

doi: 10.3390/ijms252111663

Figure Lengend Snippet: Linear regression analysis between serum protein levels, demographic and clinical variables in PSP patients.

Techniques:

Linear regression analysis between serum protein levels and age in HC.

Journal: International Journal of Molecular Sciences

Article Title: Proximity Elongation Assay and ELISA for the Identification of Serum Diagnostic Biomarkers in Parkinson’s Disease and Progressive Supranuclear Palsy

doi: 10.3390/ijms252111663

Figure Lengend Snippet: Linear regression analysis between serum protein levels and age in HC.

Techniques:

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1) Product Images from "Inhibition of TFF3 improves the infiltration and function of CD8 + T cells by downregulating the expression of PD-L1 in colorectal cancer"

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